taq dna ligase (New England Biolabs)

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New England Biolabs taq dna ligase
Taq Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1336 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/taq+ligase/Taq+DNA+Ligase%2C+High+Concentration/pm42105764-217-23-26
Average 96 stars, based on 1336 article reviews

taq dna ligase - by Bioz Stars, 2026-09

96/100 stars

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Amplification:

Article Title: Automated, modular assembly of reconstituted cell-free systems from in vitro -produced components
Article Snippet: .. Gibson assembly was performed in a 10 μL reaction using the amplified genes and the pET21a vector fragments, with T5 exonuclease, Taq ligase, and Phusion DNA polymerase (all purchased from New England Biolabs, NEB). .. Specifically, 2.5 μL of amplified genes, 2.5 μL of the pET21a backbone, and 5 μL of 2X Gibson Assembly Master Mix were combined and incubated at 50 °C for 50 min. A 2.5 μL aliquot of the assembly mix was used to transform E. coli BL21 (DE3) derivative competent cells (NEB).

Ligation:

Article Title: Construction of complex and diverse DNA sequences using DNA three-way junctions.
Article Snippet: .. The gel depicts ligation efficiency through the difference in migration of un-ligated product (lower band) compared to the ligated product (upper band). b) Comparison of 6 different 2-piece assemblies with varying toehold lengths using Taq ligase. c) DNA agarose gel of the PCR product of a 10-piece assembly of mScarlet with a toehold length of -10 using different ligases during assembly: T3 ligase (i), T4 ligase (ii), T7 ligase (iii), SplintR (iv), E. coli DNA ligase (v), Hi-T4 ligase (vi), HiFi Taq ligase (vii), Taq ligase (viii), 9°N (ix) all acquired from New England Biolabs. ..

Migration:

Comparison:

Agarose Gel Electrophoresis:

Polymerase Chain Reaction:

Article Title: Toehold-VISTA: a machine learning approach to decipher programmable RNA sensor-target interactions
Article Snippet: PCR was followed by DpnI digestion (New England Biolabs R0176S), column purification (New England Biolabs T1130S), and normalization to 100 ng/μl in ultrapure DNase/RNase-free DI water. .. Gibson assembly used unpurified insert switch or target PCR products and a custom 2× enzyme mix containing 4 U/μl T5 exonuclease (New England Biolabs M0663S), 25 U/μl Phusion polymerase (New England Biolabs M0530S), 4 U/μl Taq ligase (New England Biolabs M0208L), and 7 ng/μl ET SSB (New England Biolabs M2401S) in Gibson buffer [1 mM dNTPs (New England Biolabs N0446S), 5 mM NAD+ (New England Biolabs B9007S), 500 mM Tris–HCl (VWR 101641–844), 50 mM MgCl 2 (Sigma 63069), 50 mM DTT (Sigma 43816), 25% PEG-8000 (Sigma P2139)]. .. Each reaction received 1.5 μl of target or switch insert with 0.5 μl of corresponding linearized vector, and 2 μl of enzyme mix and was incubated at 50°C for 1 h. Transformations were performed with in-house prepared chemically competent DH5α E. coli (see Bacterial strains and reagents), followed by a 1-h recovery at 37°C in SOC media (Thermo 15544034) in deep-well plates (Thermo AB0859) shaking at 750 rpm.

Article Title: Optogenetic Proximity Labeling Maps Spatially Resolved Mitochondrial Surface Proteomes and a Locally Regulated Ribosome Pool
Article Snippet: .. Plasmid DNA constructs were cloned using in-house 2x Gibson assembly master mix (2 U T5 exonuclease (M0363S, NEB), 12.5 U Phusion polymerase (M0530S, NEB), and 200 U Taq ligase (M0208L, NEB), ISO buffer containing 1 M Tris pH 7.5, 2 M Magnesium chloride, dNTP (N0447S, NEB), PEG-8000 (V3011, Promega), and 100 mM NAD+ (B9007S, NEB) with PCR-amplified insert genes (or annealed double stranded DNA oligos) and restriction enzyme-digested backbone vectors. .. The Gibson-assembled DNA plasmids were transformed into competent bacterial cells (XL-1 Blue, 200249, Agilent; gift from Alice Y. Ting at Stanford University) and sequenced after mini-prep (silica membrane mini spin column, 1910-250, Epoch).

Modification:

Article Title: Click-code-seq reveals strand biases of DNA oxidation and depurination in human genome.
Article Snippet: Then, 2.5 μl of ddNTP mix ( Jena Biosciences, NU-1019S), 0.5 μl of 10× ThermoPol buffer (NEB, B9004S), 1.8 μl of H2O and 0.2 μl of Therminator IX (NEB (custom synthesis) 10 U per μl) was added, followed by a 10-min incubation at 60 °C. .. The repair mix modified from Zatopek et al. comprised gDNA with 1 mM NAD+ (NEB, B9007S), 1 μl of dNTP mix (NEB, N0447S), 1 μl of ENDOIV (NEB, M0304L), 5 μl of TAQ ligase (NEB, M0208S), 0.5 μl of BST DNA polymerase FL (NEB, M0328S) and 2 μl of 10× ThermoPol buffer (NEB, B9004S) in a total volume of 20 μl and was incubated for 30 min or overnight at 37 °C. .. The repair mix from Shu et al. contained 2 μl of ENDOIV (NEB, M0304L), 1 μl of BST DNA polymerase FL (NEB, M0328S), 2 μl of TAQ ligase (NEB, M0208S), 1 μl of NAD+ (NEB, B9007S), Nature Chemical Biology Article https://doi.org/10.1038/s41589-025-02052-6 1 μl of dNTP mix (NEB, N0447S, 10 mM) and 2 μl of 10× NEBuffer 3 (NEB, B7003S) in a total volume of 20 μl and was incubated for 40 min at 37 °C and 60 min at 45 °C. gDNA was purified using Pronex magnetic beads as described previously and eluted in 20 μl.

Incubation:

Article Title: Click-code-seq reveals strand biases of DNA oxidation and depurination in human genome.
Article Snippet: The repair mix modified from Zatopek et al. comprised gDNA with 1 mM NAD+ (NEB, B9007S), 1 μl of dNTP mix (NEB, N0447S), 1 μl of ENDOIV (NEB, M0304L), 5 μl of TAQ ligase (NEB, M0208S), 0.5 μl of BST DNA polymerase FL (NEB, M0328S) and 2 μl of 10× ThermoPol buffer (NEB, B9004S) in a total volume of 20 μl and was incubated for 30 min or overnight at 37 °C. .. The repair mix from Shu et al. contained 2 μl of ENDOIV (NEB, M0304L), 1 μl of BST DNA polymerase FL (NEB, M0328S), 2 μl of TAQ ligase (NEB, M0208S), 1 μl of NAD+ (NEB, B9007S), Nature Chemical Biology Article https://doi.org/10.1038/s41589-025-02052-6 1 μl of dNTP mix (NEB, N0447S, 10 mM) and 2 μl of 10× NEBuffer 3 (NEB, B7003S) in a total volume of 20 μl and was incubated for 40 min at 37 °C and 60 min at 45 °C. gDNA was purified using Pronex magnetic beads as described previously and eluted in 20 μl. ..

Purification:

Magnetic Beads:

Methylation:

Article Title: Multimodal AI for Single cfDNA Profiling and Cancer Screening
Article Snippet: .. Eluted cfDNA underwent nick repair by Taq Ligase (NEB, M0208) and dual-strand library construction, followed by enzymatic methylation conversion using a NEBNext Enzymatic Methyl-seq (EM-seq) workflow (NEB, E7120L) with unique dual indices (E7140L). ..

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